A common peptide degradation reaction where asparagine or glutamine residues lose an amide group. Accelerated by heat and alkaline pH. Detected on COAs as impurity peaks.
"Deamidation" is filed under Storage & Stability in the PeptideAtlas glossary, alongside 5 other terms in that group.
1 other glossary entry refer to this term in their own definition, which is usually the fastest way to see how it is used in practice.
A chemical compound resulting from the breakdown of a peptide through oxidation, deamidation, hydrolysis, or other processes. COAs report the percentage of degradation products to indicate product integrity.
A cycle of freezing and thawing a substance, which can damage peptide structure through ice crystal formation and aggregation. Multiple freeze-thaw cycles significantly degrade peptides; aliquoting prevents this.
The clumping together of peptide molecules, often caused by heat, agitation, or repeated freeze-thaw cycles. Aggregated peptides may lose potency and can trigger immune reactions if injected.
A degradation reaction where methionine, cysteine, or tryptophan residues in peptides react with oxygen. Exposure to light, air, or peroxides accelerates oxidation. Causes loss of potency.
Terms that reference this one: Degradation Product.
More Storage & Stability terms: Freeze-Thaw Cycle, Aggregation, Oxidation, Desiccant, Beyond-Use Date (BUD).