Free qPCR master mix samples offer reagents for up to 200 reactions at 20 µL each, targeted at new customers. Select based on assay type, starting material, and thermocycler compatibility including ROX normalization needs. Options cover probe and SYBR Green assays with features like carryover…
# Guide to Free qPCR and RT-qPCR Master Mix Samples
Free qPCR samples contain enough reagents for up to 200 rxns 20 µL . These samples suit routine testing and research with options for probe or SYBR Green assays. Selection depends on assay type, starting material, and thermocycler.
Steps to Select Your Sample
First, pick the right sample based on your needs. Then, choose the sample size on the product page. Finally, add it to your cart.
Consider assay type such as probes or SYBR Green. Factor in starting material like DNA or RNA. Match your qPCR thermocycler for best results.
Probe Assay Options
Probe assays provide highly specific detection and allow multiple targets in co-detection. They adapt well to routine testing with versatile methods.
SYBR Green assays offer a cost-efficient choice for single target detection. They use simple assay design and rely on melt curve for specificity. These suit research purposes.
Supported Thermocyclers
Match your thermocycler to its signal normalization: ROX, low ROX, or no ROX. Use the list below if unsure. Contact support if your model lacks.
ABI Prism 7500 & FAST 7500
ABI Prism 7900 & FAST 7900
ABI Step One & Plus
CFX96 & CFX384 & CFX Duet all versions
DNA Engine Opticon 1 & 2
EONIS™ Q96/Q384
Exicycler™ 96
FastGene® qFYR
iCycler iQ
LightCycler® 96 & LightCycler® 480 I & II
LightCycler Nano System
LightCycler® PRO
LineGene all versions
MiniOpticon
Mx3000P / Mx3005P/ Mx4000 with ROX normalization
Mx3000P / Mx3005P/ Mx4000 without ROX normalization
MyGo all versions
QIAquant 96 & 384
qTOWER & qTOWER³ & qTOWERiris
QuantGene 9600
QuantStudio all versions
Rotor-Gene 2000/3000/6000/Q
SmartCycler 1 & 2
Swift Spectrum 96 & ProGene
For planning qPCR in broader research, check Peptide Atlas' free peptide tools /tools including calculators for experiment setup.
ROX Options and Features
No ROX Probe Samples
No ROX DNA Probe 5X MMx dTTP Sample: Carry-over prevention not included, kit converter available, inhibitor tolerance, bacterial target, multiplex ready, one-step conversion.
No ROX Probe 2X MMx dTTP blue sample: Carry-over prevention not included, kit converter available.
No ROX Probe 2X MMx dTTP sample: Carry-over prevention not included, kit converter available.
No ROX Probe MMx UNG sample: Not compatible with carryover prevention.
Blue additive available separately. dUTP/UNG blend sold separately to enable carryover prevention by removing dUTP-containing DNA contamination from previous reactions.
Ultra Probe 4X qPCR MasterMix sample: Carry-over prevention not included, separate tube included, one-step Takyon Ultra.
Etheris Probe 2X qPCR Master Mix sample: Carry-over prevention not included, separate tube included.
SYBR Green Assay Options
SYBR options follow similar patterns for ROX, low ROX, no ROX.
ROX DNA SYBR
ROX SYBR 2X MasterMix dTTP blue sample: Carry-over prevention not included, kit converter.
Etheris SYBR 2X qPCR Master Mix blue sample: Not compatible, separate tube included.
Low ROX DNA SYBR
Low ROX SYBR 2X MasterMix dTTP blue sample: Carry-over prevention not included, kit converter.
Etheris SYBR 2X qPCR Master Mix blue sample: Not compatible, separate tube included.
No ROX DNA SYBR
No ROX SYBR 2X MasterMix dTTP blue sample: Carry-over prevention not included, kit converter.
Etheris SYBR 2X qPCR Master Mix blue sample: Not compatible.
One-Step Conversion and Notes
Convert any dTTP master mix for DNA detection to a one-step kit for RNA detection. Carryover prevention converter kit not compatible with 1-step RT-qPCR workflows. For 2-step, use first strand cDNA synthesis core kit.
Consult specialists for kit advice. See Peptide Glossary /tools/peptide-glossary for related molecular biology terms in research.
Sample Conditions
Offer valid only for new qPCR customers. Valid once per customer. For research planning, visit latest peptide news /news .
Free samples support precise qPCR and RT-qPCR experiments with matched features.